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packaging vectors pspax 2  (Thermo Fisher)


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    Thermo Fisher packaging vectors pspax 2
    Packaging Vectors Pspax 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/packaging+vectors+pspax+2/ISONICOTINIC+ACID+100GR+100GR/pm30979778-270-9-6
    Average 97 stars, based on 1 article reviews
    packaging vectors pspax 2 - by Bioz Stars, 2026-09
    97/100 stars

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    other:

    Article Title: A non‐death function of the mitochondrial apoptosis apparatus in immunity
    Article Snippet: Cells over‐expressing murine Bcl‐X L were generated by lentiviral transformation using the vector pEF1‐GWPuro‐mBcl‐X L [where mouse Bcl‐X L had been inserted into the described backbone (Weber et al , 2016 )], followed by puromycin selection.

    Produced:

    Article Title: A non‐death function of the mitochondrial apoptosis apparatus in immunity
    Article Snippet: Cells over‐expressing murine Bcl‐X L were generated by lentiviral transformation using the vector pEF1‐GWPuro‐mBcl‐X L [where mouse Bcl‐X L had been inserted into the described backbone (Weber et al , 2016 )], followed by puromycin selection. .. Lentiviruses were produced in 293FT cells (Invitrogen) using the packaging vectors psPAX.2 and psMD2.G (Addgene Plasmids, #12260 and #12259; Dr Didier Trono). .. After harvesting and filtration of the supernatants, cell lines were infected in the presence of 1 μg/ml of polybrene (Millipore, #TR‐1003‐G).

    Article Title: A non-death function of the mitochondrial apoptosis apparatus in immunity.
    Article Snippet: Cells over-expressing murine Bcl-XL were generated by lentiviral transformation using the vector pEF1-GWPuro-mBcl-XL [where mouse Bcl-XL had been inserted into the described backbone (Weber et al, 2016)], followed by puromycin selection. .. Lentiviruses were produced in 293FT cells (Invitrogen) using the packaging vectors psPAX.2 and psMD2.G (Addgene Plasmids, #12260 and #12259; Dr Didier Trono). .. After harvesting and filtration of the supernatants, cell lines were infected in the presence of 1 lg/ml of polybrene (Millipore, #TR-1003-G).



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